PROTOCOL FOR FUNGI SAMPLING IN RAPELD PLOTS
Keywords:
Amazon, Funga, conidial fungi, entomopathogenic fungi, wood fungi, Atlantic ForestAbstract
Fungi are essential organisms for the functioning of ecosystems, playing crucial roles in decomposition, nutrient cycling and establishing symbiotic relationships with various organisms. This paper presents standardized protocols for sampling fungi in RAPELD plots, covering macrofungi, decomposer microfungi and entomopathogenic fungi. The methods described aim to facilitate studies of biodiversity, ecology and biotechnological potential of Brazilian fungi. For decomposer fungi, the protocol details collection techniques in terrestrial and aquatic environments, followed by laboratory procedures for isolation and identification. Sampling macrofungi involves active searching in a defined area, with emphasis on photographic documentation and proper preservation of the samples. For entomopathogenic fungi, a systematic search for infected insects is described, with guidelines for collection and preservation. The methods are designed to be economical and applicable in places with limited resources. The implementation of these protocols in the Amazon biome, using the RAPELD method, aims to facilitate collaborative data integration throughout the biome. The results are expected to significantly expand understanding of fungal diversity, distribution and ecology in different phyto-physiognomies of the Amazon, as well as in other biomes. In addition, the protocols make it possible to collect specimens for biotechnological studies, enabling the discovery of new bioactive compounds, industrial enzymes and biological control agents. The standardization of sampling techniques also facilitates comparisons between different regions over time, contributing to a more comprehensive understanding of the dynamics of fungal communities and their role in ecosystems